FastQCFastQC Report
Sun 19 Mar 2023
SRR4896010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151305
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT2153800.0143.832861
ACTAGTA2154050.0143.816162
CTAGTAG735350.0143.66713
CTAGTAT706450.0143.459843
CTAGTAA570200.0143.229753
CTAGTAC148050.0142.686943
TAGTACG51200.0136.406254
TAGTAGT285400.0136.00284
TAGTAGG270450.0135.98674
TAGTAGA205350.0135.339684
TAGTATA222300.0135.093124
TAGTAAT223450.0134.655624
TAGTAAG205350.0134.603364
TAGTATG259350.0134.144594
TAGTACT54950.0131.94544
AGTAGTG99300.0131.456195
TAGTAGC17200.0131.441864
AGTAATG83950.0131.39255
AGTATAG80800.0131.168325
AGTAGTA86700.0129.96545
Produced by FastQC (version 0.11.5)