FastQCFastQC Report
Sun 19 Mar 2023
SRR4896010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151305
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT147710.6866065016350541No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG104650.4864489228630994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82110.3816753087079703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC80010.3719137918612191No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA57360.26662886015697446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA51950.24148133342320127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA50440.23446233797625163No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG47400.22033138025524043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA30170.14024045869832497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC26780.12448258150285524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC26560.12345994640462415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT26200.12178654351660968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG25770.1197877567337035No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC24730.11495348172388387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT23520.10932898868361296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGAGAGAGGCACTTTGC22230.10333262833489439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT647100.0135.694531
GGTATCA647200.0135.66578
GTGGTAT648100.0135.421756
TGGTATC648700.0135.374197
GTATCAA651450.0134.703269
AGCAGTG655200.0134.045142
CAGTGGT656000.0133.903764
AGTGGTA660550.0132.923835
GCAGTGG670150.0131.126973
CGTGGTA2601.200533E-1030.4611365
TCAACGC646650.027.67506810-14
CAACGCA648050.027.64194710-14
AGAGTAC648100.027.59093315-19
ATCAACG648000.027.53296910-14
GTACATG649000.027.51273220-24
CAGAGTA649950.027.51018315-19
AGTACAT652500.027.3055720-24
TACATGG654250.027.3007620-24
CGCAGAG654050.027.295915-19
GCAGAGT657100.027.20426815-19
Produced by FastQC (version 0.11.5)