FastQCFastQC Report
Sun 19 Mar 2023
SRR4896009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140135
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCTG2146450.0143.573441
AATCTGA2147850.0143.479862
ATCTGAG722750.0143.282183
ATCTGAT720950.0143.160543
ATCTGAA566650.0141.699633
TCTGATA218800.0141.564364
TCTGAGT253200.0141.21274
TCTGAGG259700.0141.143844
TCTGATG251450.0141.107424
CTGATAG75850.0140.772035
TCTGAGA202900.0140.273484
TCTGAAT204500.0139.985764
ATCTGAC151350.0139.480133
TCTGAAG192950.0139.297714
CTGATGT95900.0138.743995
CTGAGTA74850.0138.612695
CTGATGA67450.0138.341955
CTGATAT78150.0138.10315
CTGAGAT78850.0137.516275
TCTGATT249200.0137.354194
Produced by FastQC (version 0.11.5)