FastQCFastQC Report
Sun 19 Mar 2023
SRR4896009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140135
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC90040.42072112273291173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT84130.3931060423758314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC83100.38829326187366686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73380.3428755662610069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG71160.332502388867992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC46740.2183974375448278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG46490.2172292869375063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC41920.19587549383566924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG40510.18928712441037598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA39510.18461452198108996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT38990.18218476871786127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA36060.16849404360005327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC35720.166905358774096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG35710.16685863274980317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC35370.16526994792384594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT28880.13494475815777976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA27990.13078614199571523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGACATGGTGGCTCA23540.1099930611853925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT620400.0136.442446
TGGTATC622900.0135.975757
GGTATCA623250.0135.830088
AAGCAGT625200.0135.554691
GTATCAA627300.0135.005349
AGTGGTA630200.0134.377795
AGCAGTG631700.0134.126252
CAGTGGT633400.0133.787864
GCAGTGG652150.0129.85413
TCAACGC620500.027.83074810-14
CAACGCA621600.027.81161310-14
TACATGG623700.027.64741520-24
GTACATG624000.027.62950720-24
ATCAACG623900.027.60060710-14
AGAGTAC625650.027.55459815-19
CAGAGTA627400.027.49839615-19
AGTACAT627350.027.41999420-24
AACGCAG630200.027.41837310-14
CGCAGAG628650.027.39561715-19
CGACCTA14150.027.37503245-49
Produced by FastQC (version 0.11.5)