FastQCFastQC Report
Sun 19 Mar 2023
SRR4896008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3693878
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCATA3700750.0143.731282
CTGGCAT3702750.0143.653641
GGCATAG1150750.0143.436663
GGCATAT1281150.0143.392823
GGCATAA1009200.0142.772643
GCATATA383850.0142.611724
GCATAGA336700.0142.588424
GCATAGT413200.0142.55354
GCATATG411700.0142.460784
GCATAAG335500.0142.368774
GCATAGG380250.0142.182024
GCATAAT380700.0142.127444
GCATATT473950.0140.672854
GGCATAC279050.0139.974693
CATATGG144800.0139.872715
CATAGTA126050.0139.658655
CATATAG131300.0139.283865
CATAGAG110950.0139.002935
CATAATG136000.0138.493915
GCATAAA287400.0138.43824
Produced by FastQC (version 0.11.5)