FastQCFastQC Report
Sun 19 Mar 2023
SRR4896008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3693878
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG361800.979458444485714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC242880.6575203620693483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT100070.27090770187862184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100030.27079941459896617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC64820.17547953668204527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC63350.17149997915469867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG58470.1582889310367045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG52100.1410441817515359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC49750.13468230407176415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC49690.1345198731522806No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA41720.11294363268088442No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC38800.10503866126601907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1069850.0136.686051
GTGGTAT1070800.0136.588536
GGTATCA1072150.0136.470258
TGGTATC1076150.0135.95637
GTATCAA1079350.0135.565419
AGCAGTG1089950.0134.26272
CAGTGGT1091750.0134.0734
AGTGGTA1106950.0132.199455
GCAGTGG1121850.0130.513763
TCAACGC1067050.027.9667910-14
CAACGCA1069300.027.93083810-14
AGAGTAC1072100.027.76699315-19
GTACATG1073350.027.72906520-24
TACATGG1075150.027.7000520-24
ATCAACG1074950.027.67150110-14
CAGAGTA1077250.027.63157315-19
AGTACAT1078950.027.48504620-24
CGCAGAG1082950.027.41699415-19
GCAGAGT1091800.027.26597615-19
GAGTACA1071550.027.24886120-24
Produced by FastQC (version 0.11.5)