FastQCFastQC Report
Sun 19 Mar 2023
SRR4896007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980518
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCA1985750.0143.618852
CTGAGCC1986450.0143.568241
GAGCCAT663100.0143.261233
GAGCCAG636300.0142.279633
AGCCATG214550.0141.851824
GAGCCAA542700.0141.757453
AGCCAGT226100.0141.165444
AGCCATA209650.0141.114784
GCCATAG71600.0140.882265
AGCCAGA191200.0140.271564
AGCCAAT200200.0140.115464
GCCATAT71650.0139.578085
GCCAGAT67750.0139.429845
AGCCATT234050.0138.862214
GAGCCAC165050.0137.717853
GCCATTG83800.0137.2125
GCCAATA60850.0136.308555
GCCATGA65450.0135.859045
GCCAATG67050.0135.838525
GCCATGT81200.0135.75335
Produced by FastQC (version 0.11.5)