FastQCFastQC Report
Sun 19 Mar 2023
SRR4896007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980518
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG80830.4081255509922152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57010.28785398567445486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53080.26801069215225515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC48190.24332018189180812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC37760.1906571917043925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG32670.1649568446234773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC27610.13940797306563232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC27410.13839813624516414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC24820.1253207494201012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT22210.11214237891299145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG20680.10441712723640988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA20310.10254892911854374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT525750.0134.287966
GGTATCA528150.0133.650488
TGGTATC529550.0133.378727
AAGCAGT530300.0133.102811
GTATCAA530900.0132.96499
AGTGGTA536300.0131.726845
CAGTGGT537400.0131.423654
AGCAGTG537550.0131.293082
GCAGTGG550300.0128.300223
TACGCAC11250.027.90348850-54
CAACGCA524450.027.39231910-14
TCAACGC524800.027.39051210-14
GTACATG528050.027.13602320-24
ATCAACG529350.027.07074510-14
TACATGG531050.026.99628320-24
AGAGTAC531450.026.97081815-19
CAGAGTA532350.026.9306315-19
AACGCAG533450.026.89507910-14
AGTACAT532300.026.84632120-24
CGCAGAG533650.026.7894715-19
Produced by FastQC (version 0.11.5)