FastQCFastQC Report
Sun 19 Mar 2023
SRR4896006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614016
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG1616500.0143.776761
TCAATGA1616750.0143.754532
CAATGAG523150.0143.448963
CAATGAT558900.0143.316713
CAATGAA430700.0142.127183
CAATGAC113750.0141.151143
AATGATG200400.0138.789924
AATGAGT197500.0138.494674
AATGATA162800.0138.25014
AATGACG36250.0137.842244
AATGAGG183400.0137.639634
AATGAAG158100.0136.394184
AATGAAT165500.0135.38564
AATGAGA152950.0133.690264
AATGATT211650.0133.147634
ATGAGTA60700.0132.84965
ATGATAG58050.0131.720445
ATGACGC6500.0129.599535
ATGATGG70850.0129.569035
ATGAGTG68050.0129.39855
Produced by FastQC (version 0.11.5)