FastQCFastQC Report
Sun 19 Mar 2023
SRR4896006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614016
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG153190.9491231809350094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC114160.7073040168127206No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT106170.6578001705063643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61030.3781251239145089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41570.2575563067528451No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA37100.229861414013244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC30060.18624350687973354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC29190.18085322574249574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC27650.1713118085570403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC24210.14999851302589318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA24160.14968872675363812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22400.13878424997026054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT19270.11939162932709466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC18040.11177088702962051No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC17440.10805345176255997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG16870.10452188825885245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT500950.0136.086721
GTGGTAT505550.0134.698266
TGGTATC505650.0134.671637
GGTATCA507300.0134.261988
GTATCAA509200.0133.765179
CAGTGGT511450.0133.228884
AGCAGTG511700.0133.125692
AGTGGTA514150.0132.459215
GCAGTGG520300.0130.865863
TCAACGC504600.027.53913710-14
CAACGCA506850.027.4339310-14
ATCAACG506100.027.3806910-14
GTACATG505950.027.36234320-24
TACATGG506400.027.35224720-24
CAGAGTA508700.027.25422515-19
AGAGTAC508700.027.24007215-19
AGTACAT507550.027.2136720-24
GCAGAGT510100.027.15401815-19
CGCAGAG510300.027.09822715-19
ACATGGG512200.026.86540420-24
Produced by FastQC (version 0.11.5)