FastQCFastQC Report
Sun 19 Mar 2023
SRR4896005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646244
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATACA1648150.0143.829642
CGCATAC1648300.0143.816541
CATACAG530350.0143.022543
CATACAT539750.0142.91953
ATACATG184650.0141.777424
CATACAA440050.0141.218493
ATACAGT187100.0140.959924
CATACAC155600.0140.853473
ATACAGG185050.0140.692784
ATACACG51750.0140.660874
ATACATA159250.0139.750084
ATACAGA155650.0139.327994
ATACAAG154950.0138.702824
ATACAAT154150.0138.395084
ATACACT51200.0137.671884
TACAGTA54500.0137.658725
ATACATT195800.0137.564874
TACATAG54250.0136.96595
TACAGTG63750.0136.771765
TACAGGT63400.0136.731865
Produced by FastQC (version 0.11.5)