FastQCFastQC Report
Sun 19 Mar 2023
SRR4896005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646244
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC76290.463418545489004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG75590.45916644191262046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT49470.3005022341767077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49470.3005022341767077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24290.14754799410051No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC21970.13345530796163874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG20040.12173165095818116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC19990.12142792927415377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19960.12124569626373732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG19850.12057750855887706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19580.11893741146512911No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC19300.11723657003457567No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC18480.11225553441652635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC16910.10271867353806605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT391600.0136.385426
GGTATCA392650.0135.984048
AAGCAGT393100.0135.950241
TGGTATC393950.0135.571857
GTATCAA394950.0135.204449
CAGTGGT397750.0134.22644
AGTGGTA398400.0133.931055
AGCAGTG401250.0133.121542
GCAGTGG406100.0131.460753
CGTGGTA1251.32719615E-534.5593075
CGCATAC1652.481902E-630.6052651
CAACGCA391550.027.74279610-14
TCAACGC391800.027.73612210-14
AGAGTAC391450.027.68164615-19
ATCAACG392000.027.62278210-14
CAGAGTA392650.027.60071415-19
GTACATG392900.027.5973120-24
TACATGG395650.027.37273620-24
AGTACAT394950.027.3665620-24
AACGCAG400050.027.15333610-14
Produced by FastQC (version 0.11.5)