FastQCFastQC Report
Sun 19 Mar 2023
SRR4896004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572099
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCA2575950.0143.781652
AGTGGTC2577900.0143.67291
TGGTCAG918100.0143.278183
TGGTCAT749000.0143.153753
TGGTCAC217550.0142.013923
GGTCATA211400.0141.92214
GGTCATG303750.0141.866334
GGTCAGT319600.0141.859514
TGGTCAA710150.0141.819853
GGTCAAT236550.0141.534234
GGTCAGA234350.0141.203864
GTCATGA80050.0140.671755
GTCAATG102450.0140.06415
GGTCAGG359800.0140.017474
GTCATAG87200.0139.871235
GTCAGTG123700.0139.401465
GTCAGGT105950.0139.10685
GGTCATT229100.0138.845614
GTCATGG130300.0138.584495
GTCAATA62850.0138.500895
Produced by FastQC (version 0.11.5)