FastQCFastQC Report
Sun 19 Mar 2023
SRR4896004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572099
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC357801.3910817585170712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG247240.9612382727103429No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA188610.7332921477750274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCTGGTGCCAGCAGCCG184510.7173518593180123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTA170750.6638546961061763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACTCTTTCGAGGCCCTG124840.4853623441399417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA120130.46705045179054155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG93060.3618056692219079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGCCGCGGTAATTCCAG86930.33797299404105363No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76650.2980056366415134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTAAAAAGCTCGTAGTTG62000.2410482644719352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCAAATTACCCACT55600.216165862978058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG52300.2033358747077776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTTAAATCCTTTAACGAG51470.2001089382640404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCTGCCCTCTCCTGGGC49810.193655065376566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCAAGGAAGGCAGCAGGC48590.18891185759179566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT42080.16360178982224247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC41670.16200776097654096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATGATCTTGTTACTGT41160.1600249446074976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGG40790.15858643077113285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTCCCGACCCGGGGAGGT39430.15329892045368393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGATCTTGTTACTGTGATA35960.1398079933937224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA35940.13973023588905403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTTTTCCAAATGATCCTA32010.1244508862217201No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGATCCATTGGAGGGCAA29030.11286501802613352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGCCCGGCCGCCCTCCAT28960.11259286675979424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGTTCGATTCCGGAGAGG28410.11045453538141416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAGCCGCGGTAATTCCA26040.10124027107821278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1517800.0138.856631
GTGGTAT1540550.0136.692416
GGTATCA1541150.0136.643888
GTATCAA1541600.0136.52999
TGGTATC1543000.0136.49877
AGCAGTG1550050.0136.060262
AGTGGTA1550300.0135.925635
CAGTGGT1555500.0135.564444
GCAGTGG1569350.0134.386963
ACGCAGT3250.035.5186581
CGGTATC1501.1980646E-633.5993467
GGTATCC7350.029.3871848
AGGGGTA5100.028.2347475
GTATCAC7200.028.0005469
CAACGCA1539700.027.94572610-14
TCAACGC1539050.027.93226810-14
ATCAACG1539350.027.83795410-14
AGAGTAC1542600.027.80457515-19
AACGCAG1547750.027.77339610-14
CAGAGTA1545800.027.7600615-19
Produced by FastQC (version 0.11.5)