FastQCFastQC Report
Sun 19 Mar 2023
SRR4896003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697679
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG1699550.0143.838011
GACTGGA1704400.0143.428712
ACTGGAT560900.0142.72823
ACTGGAG563700.0142.325783
CTGGATA164300.0141.851734
CTGGATG198000.0141.708114
ACTGGAC144400.0141.306493
ACTGGAA451900.0141.11523
CTGGAAT162600.0140.987954
CTGGACG49400.0139.189324
CTGGAAG154400.0138.636344
CTGGATT193600.0138.457724
CTGGAGT200300.0138.139834
CTGGAGA156100.0137.403264
CTGGACT47950.0136.190924
CTGGAGG212650.0133.333664
TGGATAG62050.0131.93145
TGGAGTA61800.0131.766075
CTGGACA37800.0131.618134
TGGATGA56700.0130.792745
Produced by FastQC (version 0.11.5)