FastQCFastQC Report
Sun 19 Mar 2023
SRR4896003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697679
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG61950.36490997414705606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACAGTGGCTCA58810.3464141336495297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT48230.2840937538839793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46600.2744924099314417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT45320.26695270425092144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC40560.2389144237514866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA31850.1876090827535712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGATGCTGTGGCTCA30360.17883239410984053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC27310.1608666891679758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23270.13706949311383365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC22860.134654431138042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG22200.13076677039652373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC21040.12393391212355222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA19900.11721886175183885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC19780.11651201434429005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG19550.11515722347982156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACGGGCGCAGTGGCTCAC19030.1120942180471102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC18710.11020929162698014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCGGTGGCTCACG17240.10155041088450761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT459850.0137.155186
GGTATCA461100.0136.892678
TGGTATC462550.0136.432427
GTATCAA463200.0136.264539
AAGCAGT463850.0136.026551
AGTGGTA470400.0134.232165
CAGTGGT470500.0134.22294
AGCAGTG470300.0134.219242
GCAGTGG487300.0129.595953
CAACGCA459900.027.99642810-14
TCAACGC459550.027.97387910-14
GTACATG462500.027.73154320-24
ATCAACG462850.027.69042610-14
TACATGG464550.027.59366620-24
AGAGTAC465600.027.5936215-19
CAGAGTA466250.027.55823915-19
CGCAGAG465950.027.51726515-19
AGTACAT465200.027.496320-24
GCAGAGT469650.027.33420615-19
AACGCAG470850.027.32699610-14
Produced by FastQC (version 0.11.5)