FastQCFastQC Report
Sun 19 Mar 2023
SRR4896002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709243
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT1713850.0143.60931
GAACTTA1715400.0143.479542
AACTTAG544400.0142.743563
ACTTATG202450.0141.652744
ACTTAGG186800.0141.340474
ACTTAGT197000.0141.112694
AACTTAT594600.0140.670043
ACTTACG38600.0140.269424
ACTTAGA155150.0140.148244
AACTTAC133900.0138.784163
ACTTATA183450.0138.07364
ACTTAAG159100.0138.02644
CTTAGGT64100.0137.035875
CTTAGTG67050.0136.59065
AACTTAA484650.0136.571963
CTTATGG73900.0136.400545
CTTAGAG50050.0135.656345
ACTTAAT182000.0135.454944
CTTAGTA63000.0134.971445
CTTAGGA51200.0134.859385
Produced by FastQC (version 0.11.5)