FastQCFastQC Report
Sun 19 Mar 2023
SRR4896002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709243
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG123630.7233026550350068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC88380.5170710074576874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48610.28439490464492173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT42920.25110531387286655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA36280.21225770706681263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT30260.17703743704084207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26370.1542788240174159No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCTGTGGCTCACG25910.1515875741483218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC25710.15041746550958524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC25240.14766771020855432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC22980.13444548259083114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC21580.1262547221196752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC21450.12549415150449644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCACGGTGGCTCACG19790.11578224980298296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACGGTGGCTCAC18920.11069227722447891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT467850.0136.05641
GTGGTAT467800.0136.00996
GGTATCA469800.0135.492178
TGGTATC470750.0135.188177
GTATCAA473150.0134.532889
CAGTGGT477500.0133.417284
AGCAGTG477650.0133.341842
AGTGGTA484650.0131.400045
GCAGTGG494800.0128.734713
CAACGCA466250.027.882310-14
TCAACGC466300.027.85151710-14
AGAGTAC469650.027.57620215-19
TACATGG469000.027.57143620-24
ATCAACG469400.027.55407710-14
GTACATG469600.027.53314220-24
CAGAGTA472000.027.45720915-19
CGCAGAG472100.027.35988615-19
AGTACAT471200.027.34185820-24
GAGTACA467250.027.15079120-24
GCAGAGT478800.027.04921215-19
Produced by FastQC (version 0.11.5)