FastQCFastQC Report
Sun 19 Mar 2023
SRR4896001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118694
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGA3130950.0143.43432
TCCTGAT1046800.0143.319063
TCCTGAG1007700.0142.792483
CCTGATA309600.0142.604644
CCTGATG340850.0142.479114
CCTCCTG3154350.0142.370241
TCCTGAA866250.0141.9473
CCTGAAT322700.0141.925024
CCTGAGT366900.0141.802124
CCTGAGA289350.0141.337484
CCTGATT381800.0141.284444
CTGATAG110500.0141.002725
TCCTGAC230600.0139.660023
CTGAGTG122800.0139.426715
CTGAGTA109850.0139.21535
CCTGAGG340350.0139.028644
CTGAATG115850.0138.5935
CCTGACG70700.0138.398864
CTGATGA92750.0138.17795
CTGATTG137700.0137.67325
Produced by FastQC (version 0.11.5)