FastQCFastQC Report
Sun 19 Mar 2023
SRR4896001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118694
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG217420.6971507945313006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC160350.5141575287604363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT127990.4103961465921312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95230.30535217626352573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55900.17924169540198556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC55370.1775422660895875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA40910.13117670409472681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG37080.11889592245985017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC36620.11742094607550468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA34130.1094368347776345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT904800.0136.777681
GTGGTAT906500.0136.301486
GGTATCA908200.0136.11778
TGGTATC909850.0135.870867
GTATCAA910800.0135.721249
CAGTGGT919800.0134.568424
AGCAGTG922200.0134.1632
AGTGGTA922700.0133.978645
GCAGTGG944300.0131.04943
TCAACGC906600.027.79732110-14
CAACGCA910650.027.71797410-14
ATCAACG906700.027.69261410-14
AGAGTAC909500.027.65960715-19
GTACATG910100.027.61289220-24
CAGAGTA912300.027.59365715-19
TACATGG913250.027.53657220-24
AGTACAT913700.027.42529920-24
CGCAGAG918400.027.34452615-19
GCAGAGT922950.027.25184615-19
GAGTACA910750.027.1378320-24
Produced by FastQC (version 0.11.5)