FastQCFastQC Report
Sun 19 Mar 2023
SRR4896000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931816
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA2934900.0143.84792
CCGTGAG2937200.0143.735261
GTGAGAG940750.0142.216743
GTGAGAA817050.0142.114183
GTGAGAT1006500.0141.839653
GTGAGAC209950.0140.570623
TGAGATA313500.0139.177034
TGAGAGT316400.0139.061954
TGAGAAT295250.0138.952074
TGAGAGA282800.0138.551624
TGAGAAG292600.0137.454544
TGAGATG358800.0137.137124
TGAGAGG345200.0135.719574
TGAGACT71600.0135.050284
TGAGATT350100.0133.593834
TGAGAAA257150.0127.7604454
GAGATAG124700.0123.9069755
TGAGACG78800.0123.807114
GAGAGTA105950.0122.6616445
GAGAGTG115600.0122.5743945
Produced by FastQC (version 0.11.5)