FastQCFastQC Report
Sun 19 Mar 2023
SRR4895999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125830
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTA3128750.0143.864122
CCGAAGT3131250.0143.749271
GAAGTAG982500.0142.658813
GAAGTAT1088750.0142.505343
GAAGTAC226500.0141.361483
GAAGTAA869900.0141.14443
AAGTACG73500.0139.591744
AAGTAGT356900.0138.351264
AAGTAGA292950.0137.708044
AAGTAGG347350.0136.99374
AAGTAAG297950.0136.581194
AAGTATG374800.0136.48874
AAGTATA339750.0136.349564
AAGTAAT328800.0133.817414
AGTATAG122550.0132.71965
AAGTATT411000.0132.472894
AAGTACT77750.0131.776114
AGTAGTG121650.0130.20955
AGTATTG155500.0130.201835
AGTATGG135100.0129.983615
Produced by FastQC (version 0.11.5)