FastQCFastQC Report
Sun 19 Mar 2023
SRR4895998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144265
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTTC2152000.0143.480681
CAGTTCA2154700.0143.30092
AGTTCAT729600.0142.913763
AGTTCAG724300.0142.329273
AGTTCAA567250.0141.448033
GTTCATG248500.0140.87014
GTTCAGA192700.0140.225564
GTTCAGT280650.0140.022814
GTTCAAT207750.0139.597854
GTTCATA218750.0139.358384
GTTCAGG248500.0137.943774
TTCAGTA85500.0136.6735
GTTCATT267600.0136.600224
AGTTCAC157450.0134.487753
GTTCAAG201450.0134.385034
TCAGTAG25650.0133.613366
GTTCACG49250.0133.034874
TTCATGA74850.0132.648645
TCAGTAT34200.0131.788826
TTCAGAT75050.0131.431725
Produced by FastQC (version 0.11.5)