FastQCFastQC Report
Sun 19 Mar 2023
SRR4895997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3682004
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG285690.7759089886920275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC179900.4885926251030689No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT114670.31143366492812063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG98280.2669198621185637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94810.2574956463925623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1289600.0136.727741
GGTATCA1300450.0135.526668
TGGTATC1301750.0135.402367
GTGGTAT1302350.0135.290246
GTATCAA1306000.0134.926829
AGCAGTG1313050.0134.246172
CAGTGGT1315850.0134.075494
AGTGGTA1321950.0133.355155
GCAGTGG1356050.0130.03533
TCAACGC1296350.027.75720410-14
CAACGCA1302050.027.67550710-14
AGAGTAC1301550.027.60809315-19
ATCAACG1299050.027.58644510-14
GTACATG1304250.027.5209820-24
CAGAGTA1308400.027.4789615-19
TACATGG1307400.027.47119320-24
AGTACAT1307900.027.35279320-24
GCAGAGT1314350.027.3359415-19
CGCAGAG1311700.027.31212415-19
ACATGGG1313200.027.15248520-24
Produced by FastQC (version 0.11.5)