FastQCFastQC Report
Sun 19 Mar 2023
SRR4895996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208847
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT134770.6101373250388098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78470.3552532158180263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG75440.34153565185818663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC66390.3005640499319328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCGGTGGCTCACG47810.21644776664024265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA39940.18081831833531248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36560.16551621728440222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC32290.14618486477334103No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC29760.13473092522931648No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC28800.13038476635095142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA28630.12961513404957428No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC25160.11390558060381728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA24090.10906142435397291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT627900.0137.272811
GTGGTAT629800.0136.772066
GGTATCA630500.0136.688748
TGGTATC631750.0136.429677
GTATCAA633950.0135.965459
AGTGGTA638550.0134.909165
AGCAGTG640600.0134.563172
CAGTGGT642950.0134.059194
GCAGTGG655750.0131.410393
TCAACGC629000.027.92908910-14
CAACGCA631450.027.85721610-14
ATCAACG629250.027.82644810-14
GTACATG629150.027.80609720-24
AGAGTAC629950.027.8054515-19
CAGAGTA631950.027.72656615-19
TACATGG634200.027.6073920-24
AGTACAT634400.027.51924120-24
CGACCTA6400.027.44969245-49
CGCAGAG637650.027.43806615-19
AACGCAG642800.027.35861610-14
Produced by FastQC (version 0.11.5)