FastQCFastQC Report
Sun 19 Mar 2023
SRR4895995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243076
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1245950.0143.664831
AATGGAA1259950.0142.06852
ATGGAAT462150.0141.554053
ATGGAAG386450.0141.522063
ATGGAAC84000.0138.771423
TGGAATA140100.0138.655244
TGGAATG153400.0138.649284
ATGGAAA341800.0138.186083
TGGAAGT146750.0137.719924
TGGAAAT137100.0135.01974
TGGAATT175000.0134.331444
TGGAAGA115550.0134.09264
GGAATAG47200.0132.711875
TGGAACT32750.0131.46874
TGGAAGG134350.0131.298864
GGAATTG61250.0130.951835
GGAATGT62300.0130.131625
GGAAGTA45100.0128.354775
TGGAAAG123350.0128.18164
GGAATAT55150.0128.072535
Produced by FastQC (version 0.11.5)