FastQCFastQC Report
Sun 19 Mar 2023
SRR4895995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243076
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG73890.5944125701083441No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC56130.4515411768870125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47820.3846908797209503No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT40870.32878118473850354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG27400.22042095575813547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG24540.19741351293082643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG22590.18172662009402482No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA18040.14512387014148773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA16980.13659663608661096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC16550.13313747510208546No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC15590.1254146970901216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA15250.12267954654421774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC13360.10747532733316387No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC13000.10457928557867742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT313350.0136.815231
GTGGTAT314400.0136.189246
GGTATCA315300.0135.846188
TGGTATC315550.0135.761377
GTATCAA316050.0135.660499
CAGTGGT319800.0133.872484
AGCAGTG320650.0133.640642
AGTGGTA324950.0131.679035
GCAGTGG330800.0129.561873
TCAACGC314950.027.83494810-14
AGAGTAC314250.027.78239615-19
CAACGCA315650.027.76865810-14
CAGAGTA314800.027.75672715-19
GTACATG314800.027.72013520-24
TACATGG315150.027.67563820-24
ATCAACG315650.027.66373310-14
AGTACAT315850.027.5322420-24
GCAGAGT319500.027.35291915-19
CGCAGAG319700.027.30427715-19
GAGTACA315000.027.1676820-24
Produced by FastQC (version 0.11.5)