FastQCFastQC Report
Sun 19 Mar 2023
SRR4895994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801904
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA802550.0143.882931
TGCCTAA803250.0143.757552
GCCTAAG302450.0143.618673
GCCTAAT238850.0143.366553
GCCTAAA216950.0142.572513
CCTAATG101800.0142.51434
CCTAAGT99800.0142.484534
CCTAAGG118050.0142.352814
CCTAAGA81950.0141.18814
CCTAATA68950.0141.180134
CTAAGTG38400.0140.812075
CTAATAG28850.0140.75525
GCCTAAC48750.0140.750343
CCTAAAG89800.0140.151024
CTAATGA25250.0140.00755
CTAATGG40300.0139.354425
CCTAACG19900.0139.296074
CTAAAGT31150.0139.145655
CTAAATG33850.0139.10745
CTAAGGT39550.0139.084295
Produced by FastQC (version 0.11.5)