FastQCFastQC Report
Sun 19 Mar 2023
SRR4895994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801904
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG34570.4310989844170873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29890.37273788383646916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT23710.29567130230052474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA21490.26798719048664177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21180.2641213910892077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20630.25726271473892137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG17260.21523773419262157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA14390.17944791396476387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC11890.14827211237255333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC11140.13891937189489018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG9780.12195973582872764No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC9630.12008918773319499No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA9010.11235758893832679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT206950.0135.31561
GTGGTAT207700.0134.983386
GGTATCA208000.0134.78878
TGGTATC208450.0134.532267
AGCAGTG208650.0134.359422
GTATCAA208700.0134.353359
CAGTGGT211300.0132.862274
AGTGGTA212100.0132.284995
GCAGTGG212700.0131.860583
TATATCG552.3101165E-1028.79922590-94
AACGCGA1500.027.83994745-49
TCAACGC207200.027.61428610-14
AGAGTAC207450.027.58519715-19
CAACGCA207700.027.58247810-14
ATCAACG207500.027.49107710-14
TACATGG208300.027.41664320-24
GTACATG208400.027.3827620-24
CAGAGTA209300.027.37577415-19
CGCAGAG209800.027.20071215-19
AGTACAT209650.027.18515420-24
Produced by FastQC (version 0.11.5)