FastQCFastQC Report
Sun 19 Mar 2023
SRR4895993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856710
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACT1859750.0143.763841
AAGACTA1860800.0143.682722
AGACTAG616850.0143.591463
AGACTAT638900.0143.357653
GACTAGT227650.0142.987924
GACTAGA162750.0142.938254
AGACTAA480000.0142.893
GACTAGG216100.0142.667284
GACTAAG161350.0142.661294
AGACTAC130550.0142.566073
GACTATG225750.0142.46914
GACTATA182700.0142.029544
GACTAAT182450.0142.026864
GACTATT224600.0141.371324
GACTACT46550.0140.751884
GACTACG43450.0140.188724
ACTAGTG81100.0139.738595
ACTATAG68450.0139.476995
ACTAGAT62600.0139.284355
ACTAGGT74650.0139.081045
Produced by FastQC (version 0.11.5)