FastQCFastQC Report
Sun 19 Mar 2023
SRR4895993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856710
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG138180.74421961426394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96010.5170974465586978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT69830.37609535145499295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58140.31313452289264343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA44460.23945581162378615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33750.18177313635408868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA28990.1561363917897787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC22230.11972790581189308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG20280.10922545793365684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA18820.10136208670174662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT519550.0136.264636
GGTATCA520750.0136.005948
AAGCAGT523050.0135.708651
TGGTATC522100.0135.61297
GTATCAA523300.0135.401879
CAGTGGT532950.0133.078934
AGCAGTG535400.0132.491362
AGTGGTA538900.0131.452035
GCAGTGG539900.0131.360383
TCAACGC517950.027.91891510-14
CAACGCA520900.027.78291710-14
ATCAACG520450.027.67413110-14
GTACATG522350.027.61683520-24
AGAGTAC523350.027.54770315-19
CAGAGTA524050.027.54662715-19
TACATGG523950.027.532520-24
AGTACAT524900.027.41408520-24
CGCAGAG529600.027.2035715-19
GAGTACA522050.027.13069220-24
GCAGAGT534200.027.01784115-19
Produced by FastQC (version 0.11.5)