FastQCFastQC Report
Sun 19 Mar 2023
SRR4895992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178304
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG126990.5829764807850512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC86720.3981078857680103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80490.3695076536608297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76920.3531187566106475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA47650.218748163709014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA44310.20341513397579034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT41150.18890843518627334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG30140.13836452579621578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC27880.1279894817252321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTGCGGTGGCTCAC27550.12647454166176988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC25540.11724717945704548No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACGGTGGCTCAC24540.11265645199200847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT615900.0133.047061
GTGGTAT618200.0132.56096
GGTATCA618550.0132.509168
TGGTATC618950.0132.388637
GTATCAA622850.0131.649669
AGCAGTG628000.0130.472342
CAGTGGT628500.0130.468664
AGTGGTA629000.0130.319155
GCAGTGG644100.0127.2557453
TCAACGC617850.027.08375210-14
CAACGCA619400.027.03457610-14
AGAGTAC618000.027.01044515-19
ATCAACG618800.026.98166510-14
GTACATG618500.026.9719420-24
CAGAGTA619550.026.96146415-19
TACATGG620050.026.89987220-24
AGTACAT621700.026.7914220-24
CGCAGAG624750.026.7024815-19
GCAGAGT628700.026.56906915-19
GAGTACA619100.026.46200620-24
Produced by FastQC (version 0.11.5)