FastQCFastQC Report
Sun 19 Mar 2023
SRR4895991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650293
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGAGG2667650.0143.060351
TTGAGGA2672250.0142.814092
TGAGGAT932500.0142.138813
TGAGGAG853300.0140.945113
TGAGGAA736350.0140.156883
TGAGGAC182850.0140.101333
GAGGATA294000.0136.211874
GAGGAGT321300.0135.484224
GAGGATG313600.0134.976674
GAGGATT356900.0133.1264
GAGGAAT290050.0132.307864
GAGGACT71600.0131.329254
AGGATAG103200.0130.743845
GAGGAGA259800.0129.394564
AGGATTG123700.0128.691645
AGGAGTG101900.0126.2646265
GAGGACG62150.0125.9272464
AGGATAT112850.0125.178215
GAGGAAG263950.0125.096074
AGGATGT125350.0125.044745
Produced by FastQC (version 0.11.5)