FastQCFastQC Report
Sun 19 Mar 2023
SRR4895991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650293
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT108070.40776623565771786No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91910.3467918452789937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG74090.27955399648265306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG60820.22948406081893585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA33570.12666524040926797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32530.12274114597895402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCTCGGGTCGCTGCAGAC31560.119081173289142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCGCCTTTCCCTTTAGCC27720.10459220923875209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1206300.0138.666721
GTGGTAT1222250.0136.798936
GGTATCA1222200.0136.780968
TGGTATC1225400.0136.500157
GTATCAA1227650.0136.199779
AGTGGTA1228150.0136.159335
AGCAGTG1232600.0135.72372
CAGTGGT1233350.0135.677954
GCAGTGG1250050.0133.852833
TCAACGC1221900.027.9467810-14
CAACGCA1225350.027.88924810-14
ATCAACG1222150.027.84326710-14
AGAGTAC1225200.027.82054715-19
CAGAGTA1226750.027.80652415-19
TACATGG1225550.027.78194820-24
GTACATG1227050.027.74329220-24
AACGCAG1234250.027.65547410-14
CGCAGAG1232350.027.61239615-19
GCAGAGT1235550.027.60731115-19
AGTACAT1230550.027.60236420-24
Produced by FastQC (version 0.11.5)