FastQCFastQC Report
Sun 19 Mar 2023
SRR4895990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261378
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGTA1264200.0143.675082
ATCCTGT1265800.0143.493481
CCTGTAG405350.0143.466863
CCTGTAT445800.0143.418293
CTGTATG144200.0142.801394
CCTGTAA329650.0142.470843
CTGTATA132100.0142.092094
CTGTAGT159200.0142.009774
CTGTAGA110000.0141.970634
CTGTAAT130250.0141.843874
CTGTAGG132150.0141.221074
CCTGTAC89550.0140.462043
CTGTAAG104000.0139.984344
CTGTATT167550.0139.358734
CTGTACG26750.0138.34744
CTGTACT35050.0138.247964
CTGTAAA96550.0136.393334
TGTAGTA46600.0132.875295
CTGTACA23200.0131.585954
GTAGTAG14500.0130.592866
Produced by FastQC (version 0.11.5)