FastQCFastQC Report
Sun 19 Mar 2023
SRR4895990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261378
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG109050.8645306957945992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT94540.7494977714848364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC71790.5691394649343813No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40260.3191747438119263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37530.2975317470258717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA28980.22974873511350286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC19660.15586128821019551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19440.15411716392707023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC18400.14587221277047802No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC17660.14000561290905658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA16750.1327912806470384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT15400.12208869981876963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG15320.12145447280672407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14650.11614282158084253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG14000.11098972710797239No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATCTTAGTTCAACTTTA13900.11019694334291545No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC13740.10892848931882432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT366700.0134.445571
GGTATCA369850.0133.485728
GTGGTAT369650.0133.460566
GTATCAA370450.0133.288969
TGGTATC370600.0133.215587
AGCAGTG373250.0132.111862
CAGTGGT373950.0132.022174
AGTGGTA377450.0130.721665
GCAGTGG384600.0128.306723
TCAACGC369600.027.22947910-14
CGACCTA7250.027.21064645-49
AGAGTAC370300.027.14689615-19
CAGAGTA371000.027.11896515-19
ATCAACG370000.027.1105310-14
GTACATG370350.027.10046220-24
CAACGCA372300.027.07842310-14
TACATGG371500.027.05920820-24
AGTACAT371550.026.95480320-24
GCAGAGT376300.026.70256415-19
TACGCAC7550.026.70160550-54
Produced by FastQC (version 0.11.5)