FastQCFastQC Report
Sun 19 Mar 2023
SRR4895989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638036
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA1639350.0143.881422
AGTCACT1640200.0143.806851
TCACTAG465800.0143.752693
TCACTAA660850.0143.368093
CACTAGA274300.0143.055054
TCACTAT382200.0143.001573
CACTAAG183950.0142.316944
CACTAGG121250.0142.218554
TCACTAC137500.0141.905463
CACTATG123300.0141.722634
CACTATA216700.0141.67424
CACTACG39200.0141.612244
CACTAAA406300.0141.076054
CACTAGT63450.0141.049654
ACTAGAA163300.0139.502765
CTATAAG41750.0139.34376
ACTAGGA68250.0139.041755
ACTAGAG78050.0139.018595
ACTATAG76600.0138.642295
ACTATGA68000.0138.494115
Produced by FastQC (version 0.11.5)