FastQCFastQC Report
Sun 19 Mar 2023
SRR4895989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638036
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70260.42892830194208187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA53110.32422974830834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG48170.2940716809642767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG46730.28528066538220165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA42350.25854132632005644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC41030.25048289536982093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCACGGTGGCTCAC28150.1718521448857046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGTGGTGGCTCAC28070.17136375513114485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCTCGGGTCGCTGCAGAC27160.16580832167302795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCACACCTGGGAACTCCG23060.14077834675184184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT23060.14077834675184184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA22950.1401068108393222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA20750.12667609258892967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG20510.1252109233252505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC18980.11587046926929566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCCAACACAGCCACACC18870.11519893335677604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC18820.11489368976017622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCACGGTGGCTCACG18640.11379481281241681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG17760.10842252551225981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT727050.0137.734651
GGTATCA733950.0136.36628
GTGGTAT734100.0136.357966
TGGTATC735700.0136.100547
GTATCAA735500.0135.980939
AGCAGTG736900.0135.85792
AGTGGTA739550.0135.353095
CAGTGGT740250.0135.248664
GCAGTGG755450.0132.521913
TCAACGC732650.027.801210-14
CAACGCA733700.027.7888910-14
AGAGTAC734200.027.70328315-19
ATCAACG732850.027.70322810-14
CAGAGTA735900.027.6725515-19
TACATGG735450.027.6424920-24
GTACATG735350.027.64037520-24
AGTACAT736100.027.52809320-24
CGCAGAG737550.027.5188815-19
GCAGAGT739400.027.50845315-19
AACGCAG740800.027.48756610-14
Produced by FastQC (version 0.11.5)