FastQCFastQC Report
Sun 19 Mar 2023
SRR4895988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103479
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGGA1107950.0143.414831
GCAGGAA1110500.0143.085512
CAGGAAT390900.0142.010443
CAGGAAG347650.0140.333943
CAGGAAC89700.0138.380953
AGGAAGT138000.0137.373614
AGGAATG133350.0135.684744
AGGAATA116550.0135.598144
AGGAACT35550.0134.278184
AGGAATT153300.0132.868594
CAGGAAA319950.0131.847813
GGAAGTA43950.0130.89395
GGAATAG38700.0130.232275
AGGAAAT124900.0130.22234
AGGAACG29050.0129.872364
GGAACTA8050.0129.689155
GGAATTG52900.0128.619755
GGAATGT53750.0127.6573945
AGGAAGA103550.0126.060564
GGAATAT43750.0125.0740055
Produced by FastQC (version 0.11.5)