FastQCFastQC Report
Sun 19 Mar 2023
SRR4895988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103479
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT82090.7439199114799647No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA48650.44087834929346187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47050.42637875301659567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA38100.34527163634287555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35550.32216290477662013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC34800.31536621902183914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC29530.2676081737849112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA29230.2648894994829988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC27290.24730873899729855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21850.19801011165595356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA20780.18831350664579935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT17750.16085489619648402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG17610.15958618152225823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG16690.1512489136630602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC16150.14635529991961785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG15310.13874301187426313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGCGCGGTGGCTCAGG14030.12714333485277018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC13650.12369968073701448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC13500.12234034358605828No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC13300.12052789405145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11820.10711576749534879No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG11660.10566580786766219No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC11350.10285651108901937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG11320.10258464365882813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT310950.0136.747711
GGTATCA313150.0135.65138
GTGGTAT313000.0135.647326
TGGTATC313400.0135.566097
GTATCAA314400.0135.186819
AGCAGTG315800.0134.605822
CAGTGGT317800.0133.734454
AGTGGTA318150.0133.474185
GCAGTGG325500.0130.57243
TCAACGC313250.027.65609410-14
AGAGTAC313550.027.56892815-19
CAACGCA314650.027.56507910-14
ATCAACG313700.027.49248110-14
CAGAGTA314550.027.4904415-19
GTACATG315350.027.41588420-24
TACATGG315400.027.38871220-24
CGCAGAG316800.027.21337515-19
AGTACAT316350.027.2017720-24
GCAGAGT318100.027.15648515-19
GAGTACA313950.027.03361120-24
Produced by FastQC (version 0.11.5)