FastQCFastQC Report
Sun 19 Mar 2023
SRR4895987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225628
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA2226950.0143.91272
AGATCGC2227200.0143.896551
ATCGCAT758050.0143.791053
ATCGCAA585300.0143.606353
TCGCAGT263950.0143.590844
ATCGCAG715100.0143.506643
TCGCATG241250.0143.283724
TCGCAAT224000.0143.099994
TCGCATA228100.0143.021484
TCGCATT275200.0142.953494
ATCGCAC173600.0142.921663
TCGCAAG181350.0142.888354
TCGCAGA194200.0142.516984
CGCATAG78200.0142.434785
CGCAGTA80700.0142.304825
TCGCAGG244000.0141.816394
CGCATGA65900.0141.814875
CGCAATA64800.0141.777775
CGCATTG97100.0141.775485
CGCATTA77800.0141.501285
Produced by FastQC (version 0.11.5)