FastQCFastQC Report
Sun 19 Mar 2023
SRR4895987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225628
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT94530.42473405259099906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG91090.4092777409342442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81860.3678063000645211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC58720.26383564548972244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55390.24887357635687543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA38040.170918050995045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG30230.135826831797587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCTCGGGTCGCTGCAGAC29480.13245699640730615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA26390.11857327459934904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACTCTTAGCGGTGGATCA26260.11798916979836702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC24930.11201332837293564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC24300.10918266664509972No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC24230.10886814867534017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA23810.1069810408567829No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22520.10118492398549983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT763450.0137.071951
GTGGTAT767100.0136.489446
GGTATCA768250.0136.313268
TGGTATC770000.0136.040867
GTATCAA770400.0135.954579
AGCAGTG775250.0135.107092
CAGTGGT776400.0134.941024
AGTGGTA777000.0134.759675
GCAGTGG785850.0133.284683
TCAACGC766100.027.8360110-14
AGAGTAC767550.027.73039215-19
ATCAACG766800.027.7073110-14
CAACGCA770750.027.6830210-14
CAGAGTA769100.027.6782515-19
GTACATG767850.027.67067320-24
TACATGG768850.027.61408420-24
AGTACAT772350.027.4311520-24
CGCAGAG775200.027.37500615-19
GCAGAGT778450.027.3384115-19
GAGTACA767450.027.2103920-24
Produced by FastQC (version 0.11.5)