FastQCFastQC Report
Sun 19 Mar 2023
SRR4895986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484942
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCA1486800.0143.815281
GAGTCAA1487000.0143.795932
AGTCAAG488200.0143.424123
AGTCAAT516450.0143.316163
GTCAATG176200.0142.56914
GTCAATA151750.0142.291234
GTCAAGA141050.0141.600164
GTCAAGT173050.0141.461324
GTCAATT182900.0141.086244
AGTCAAC118800.0141.029623
AGTCAAA376750.0140.769583
GTCAAAT144600.0140.11554
TCAAGTA50400.0139.713615
GTCAACG39600.0139.272034
TCAATAG54700.0138.865885
GTCAAGG172100.0138.853474
GTCAACT40050.0138.606054
TCAATGA50500.0137.155765
TCAATTG68900.0136.579885
TCAAGAT54900.0136.392765
Produced by FastQC (version 0.11.5)