FastQCFastQC Report
Sun 19 Mar 2023
SRR4895986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484942
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT63610.42836689917855375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53600.3609568589210892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43120.2903817118783091No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG31110.2095031321088635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC29540.1989303285919585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC27660.18626990145069638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26290.17704395188498945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA25990.1750236709581923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC24660.166067092182725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC17910.12061077132978931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA17260.1162334959883955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15330.1032363553593339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC15300.1030343272666542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG15200.10236090029105513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT368450.0136.200016
TGGTATC370450.0135.484137
GGTATCA370350.0135.423518
GTATCAA371000.0135.122279
AAGCAGT372450.0134.876791
AGTGGTA374000.0134.178865
AGCAGTG375500.0133.82332
CAGTGGT376300.0133.463414
GCAGTGG383700.0130.907073
TCAACGC367700.027.80355610-14
CAACGCA369300.027.70259510-14
CGTGGTA1306.753044E-427.6916985
AGAGTAC368450.027.67046415-19
ATCAACG368950.027.63519510-14
CAGAGTA369600.027.6233315-19
GTACATG370050.027.54248220-24
TACATGG371000.027.4564320-24
TGTCGTA1050.00797264927.4298134
AGTACAT373350.027.24504320-24
GCAGAGT375750.027.19420415-19
Produced by FastQC (version 0.11.5)