FastQCFastQC Report
Sun 19 Mar 2023
SRR4895985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987367
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC1988450.0143.919981
CGTATCA1989450.0143.847662
GTATCAG651500.0143.546553
GTATCAA522800.0143.283513
GTATCAT680900.0143.100833
GTATCAC142300.0142.734733
TATCAGT244300.0142.37874
TATCAGG219650.0142.262344
TATCAAG179650.0142.196144
TATCATG237200.0141.965944
TATCAGA180600.0141.527914
TATCATA201750.0141.073264
TATCAAT197500.0140.75514
TATCACG48500.0140.585224
ATCAGTA70800.0140.135245
ATCATGA64750.0139.774195
ATCAGGT78750.0139.428245
ATCAGTG80850.0139.368875
ATCAGAT63450.0138.893285
ATCATAG72250.0138.618355
Produced by FastQC (version 0.11.5)