FastQCFastQC Report
Sun 19 Mar 2023
SRR4895985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987367
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG93150.468710610571676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT88700.4463191750693254No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79480.39992613342175853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC59110.2974287084368413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37940.1909058568447599No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA34980.17601177839825258No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC28830.14506631135567813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC28080.14129247391146174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA27830.14003452809672295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC24300.12227233319261113No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC23820.11985707722831263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCATCTTTTGGGGGGAG20390.10259806065009633No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT20260.10194392882643215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19960.1004343938487456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT531450.0135.779161
GGTATCA533750.0135.296728
GTGGTAT534150.0135.1286
TGGTATC535250.0134.944477
GTATCAA535400.0134.802589
AGCAGTG541050.0133.444762
CAGTGGT543050.0132.983054
AGTGGTA546300.0132.149055
GCAGTGG550550.0131.142123
ACGTATC1905.7207217E-934.1604771
CGTATCA2003.4579534E-728.8023512
TCAACGC532500.027.62931810-14
CAACGCA535050.027.52724310-14
AGAGTAC534050.027.48972915-19
ATCAACG534250.027.45255310-14
CAGAGTA535850.027.40813615-19
TACATGG535650.027.40692520-24
GTACATG535850.027.3940120-24
AGTACAT537500.027.21079420-24
CGCAGAG540600.027.10072115-19
Produced by FastQC (version 0.11.5)