FastQCFastQC Report
Sun 19 Mar 2023
SRR4895984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727382
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG1729650.0143.808522
ACATCGG512350.0143.606523
ACATCGT650950.0143.568633
ACATCGA413700.0143.530093
CATCGAT177550.0143.14844
CATCGTA150250.0143.04164
ACATCGC156150.0142.98563
AAACATC1740450.0142.916141
CATCGTG184500.0142.907324
CATCGGT209800.0142.901814
CATCGGA117250.0142.833274
CATCGTT306800.0142.779654
CATCGAA103600.0142.262564
ATCGATA46950.0141.546335
ATCGTAG45150.0141.44855
ATCGCGA10050.0141.134325
ATCGATG49900.0141.114235
ATCGAGT44700.0140.93965
CATCGGG175800.0140.928334
ATCGGAT46650.0140.758835
Produced by FastQC (version 0.11.5)