FastQCFastQC Report
Sun 19 Mar 2023
SRR4895984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727382
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63760.36911349082021233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53860.31180132709499114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50260.2909605402858198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32940.19069319930391773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG30020.17378900555870097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA24100.1395174894725081No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA21210.12278696895070111No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC20630.11942928663144575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT19560.11323494166316425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG19250.11144031835459672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC17540.10154094462024035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT812850.0138.7891
GTGGTAT823850.0136.945116
GGTATCA824450.0136.801798
AGCAGTG825050.0136.782382
TGGTATC825300.0136.748127
GTATCAA825700.0136.4949
AGTGGTA828550.0136.211735
CAGTGGT829850.0136.062234
GCAGTGG848650.0132.983253
GGTATCC4050.028.4439988
ACGCAGT2553.2050593E-928.2798731
TCAACGC822400.027.93188910-14
CAACGCA823900.027.91424210-14
AGAGTAC823100.027.87074715-19
ATCAACG822800.027.81855210-14
CAGAGTA826500.027.77351615-19
GTACATG825050.027.74013720-24
TACATGG826500.027.70366720-24
AACGCAG830400.027.67666810-14
CGCAGAG828000.027.6362515-19
Produced by FastQC (version 0.11.5)