FastQCFastQC Report
Sun 19 Mar 2023
SRR4895983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477450
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCG1478250.0143.92161
CGCTCGA1478450.0143.902132
GCTCGAT500000.0143.740313
GCTCGAA394350.0143.524833
GCTCGAG473800.0143.452473
CTCGAGT167800.0143.441734
CTCGAAT146400.0143.36024
CTCGAGA137000.0143.263764
CTCGATG167950.0143.1854
CTCGATA153650.0143.156054
GCTCGAC114000.0143.115333
CTCGATT170750.0142.945364
TCGAGTA49250.0142.683795
CTCGAAG128300.0142.484334
TCGATAT52700.0142.360065
CTCGACG39250.0141.981694
TCGATAG53500.0141.980835
CTCGAGG157600.0141.760964
TCGAATG47450.0141.723465
TCGAGAT52850.0141.683555
Produced by FastQC (version 0.11.5)