FastQCFastQC Report
Sun 19 Mar 2023
SRR4895983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477450
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA124000.8392839013164575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG98060.6637111238959017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC71770.4857693999796947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT69720.4718941419337372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC68190.46153846153846156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40850.27648989813530067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGTGGTGGCTCA39720.2688415851636265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38470.26038106196487193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCTGCGCGGTGGCTCAG34430.233036650986497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG33660.22782496869606417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC27820.18829740431148262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA25150.1702257267589428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCGC22700.15364310128938372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGTGCAGTGGCTCA21670.14667163017360993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTTTTCCAAATGATCCTA17640.11939490338082506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC16360.11073132762530036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCGCA15030.10172933094182546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT687750.0138.462511
GTGGTAT694400.0136.978136
GGTATCA694250.0136.893658
TGGTATC695350.0136.832417
GTATCAA695500.0136.652249
AGCAGTG700150.0136.00992
CAGTGGT700500.0136.000084
AGTGGTA705500.0134.853595
GCAGTGG714050.0133.3323
GTATCCA3700.033.081659
CGATCGT200.00628640228.7995270-74
TCAACGC694700.027.9526910-14
CAACGCA696550.027.9073910-14
AGAGTAC696150.027.85027115-19
TACATGG695850.027.8372620-24
ATCAACG694650.027.83653810-14
CAGAGTA697150.027.8227215-19
GGTATCC4400.027.8177198
GTACATG696800.027.81170520-24
AGTACAT699200.027.64004120-24
Produced by FastQC (version 0.11.5)